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Mashup Score: 0Advanced Flow Cytometry in Immuno-oncology - 2 year(s) ago
Many research efforts have resulted in the discovery of immunotherapies such as chimeric antigen receptor (CAR) T cells, checkpoint inhibitors, and antibodies, which have revolutionized cancer treatment. Unlike traditional treatments (e.g., chemotherapy and radiotherapy), immunotherapy harnesses the body’s own immune system to fight cancer. This approach has a greater level of target specificity,…
Source: secure.wiley.comCategories: General Medicine Journals and Societies, Latest HeadlinesTweet
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Mashup Score: 0Advanced Flow Cytometry in Immuno-oncology - 2 year(s) ago
Many research efforts have resulted in the discovery of immunotherapies such as chimeric antigen receptor (CAR) T cells, checkpoint inhibitors, and antibodies, which have revolutionized cancer treatment. Unlike traditional treatments (e.g., chemotherapy and radiotherapy), immunotherapy harnesses the body’s own immune system to fight cancer. This approach has a greater level of target specificity,…
Source: secure.wiley.comCategories: General Medicine Journals and Societies, Latest HeadlinesTweet
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Mashup Score: 2EVOS Cell Imaging Systems - 2 year(s) ago
Imaging is an integral tool for scientist across a range of research subjects, from analyzing the expression of individual proteins or genes in single cells to the screening of drugs in multiwell plates. For the last decade and a half, the family of EVOS Cell Imaging Systems has been a leader in microscopy technology utilized by scientists around the world. Providing self-contained and easy to…
Source: secure.wiley.comCategories: Hem/Onc News and Journals, Latest HeadlinesTweet
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Mashup Score: 0Optimizing Flow Cytometric Analysis of Immune Cells in Samples Requiring Cryopreservation from Tumor-Bearing Mice - 2 year(s) ago
Most shared resource flow cytometry facilities do not permit analysis of radioactive samples. We are investigating low-dose molecular targeted radionuclide therapy (MTRT) as an immunomodulator in combination with in situ tumor vaccines and need to analyze radioactive samples from MTRT-treated mice
Source: The Journal of ImmunologyCategories: Allergy-Immunology, Latest HeadlinesTweet
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Mashup Score: 8Flow cytometric analysis of CD4+ T cell reactivation following anti-PD1 immunotherapy in a transgenic mouse model: STAR Protocols - 2 year(s) ago
Summary This protocol uses the Tg4 Nr4a3-Tocky mouse model to recalibrate T cell activation thresholds and reveals the role that immune checkpoints play in controlling T cell activation. The example approach here uses flow cytometry to characterize quantitative and qualitative changes in splenic CD4+ T cells reactivated in the presence of anti-PD1 immunotherapy. The…
Source: star-protocols.cell.comCategories: General Medicine News, Latest HeadlinesTweet
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Mashup Score: 1An optimized protocol to analyze membrane protein degradation in yeast using quantitative western blot and flow cytometry: STAR Protocols - 2 year(s) ago
Summary Membrane proteins (MPs) are essential in many cellular functions. To maintain proteostasis, MPs are downregulated via ubiquitination and degradation. Here, we describe an optimized protocol to analyze MP degradation using quantitative western blot and flow cytometry-based approaches. We use the degradation of Ypq1, a vacuole membrane lysine transporter, to…
Source: star-protocols.cell.comCategories: General Medicine News, Latest HeadlinesTweet
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Mashup Score: 4Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry: STAR Protocols - 2 year(s) ago
Summary This protocol describes an ex vivo approach to identify and quantify the proportions of proliferating neural stem cells and progenitors of the mouse subventricular zone. It uses ethynyl deoxyuridine (EdU) incorporation to identify dividing cells, combined with multicolor flow cytometry for 4 cell surface antigens to distinguish between 8 phenotypically distinct…
Source: star-protocols.cell.comCategories: General Medicine News, Latest HeadlinesTweet
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Mashup Score: 15
Inflammation and dysfunction of the extrahepatic biliary tree are common causes of human pathology, including gallstones and cholangiocarcinoma. Despite this, we know little about the local regulat…
Source: www.science.orgCategories: Allergy-Immunology, Latest HeadlinesTweet
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Mashup Score: 12
Tuft cells in the biliary tree are bile-sensitive cells that, together with the microbiome, regulate biliary inflammatory tone.
Source: Science ImmunologyCategories: Allergy-Immunology, Latest HeadlinesTweet
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Mashup Score: 2SIGLEC1 enables straightforward assessment of type I interferon activity in idiopathic inflammatory myopathies - 2 year(s) ago
Objective To evaluate sialic acid binding Ig-like lectin 1 (SIGLEC1) expression on monocytes by flow cytometry as a type I interferon biomarker in idiopathic inflammatory myopathies (IIM). Methods We performed a retrospective analysis of adult and paediatric patients with the diagnosis of IIM. SIGLEC1 expression was assessed by flow cytometry and was compared with Physician Global Assessment or…
Source: RMD OpenCategories: Latest Headlines, RheumatologyTweet
Free article collection now available – Advanced Flow Cytometry in Immuno-oncology: Advancing the Development of Immunotherapies. Access it here: https://t.co/6JmkuwZgYW #FlowCytometry #ImmunoOncology https://t.co/kKnTLDtSQK