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Mashup Score: 81Complement Terminal Pathway Activation and Intrarenal... : Journal of the American Society of Nephrology - 1 day(s) ago
ied a cohort of 42 patients diagnosed with C3 glomerulopathy. We performed centralized extensive characterization of histological parameters. Kidney C5b-9 staining was performed as a marker of terminal pathway activation, intra-renal immune response was characterised through transcriptomic analysis. Results: Eighty-eight percent of biopsies showed C5b-9 deposits in glomeruli. Biopsies were grouped according to the amount of C5b-9 deposits (no or low n=15/42, 36%, intermediate n=15/42, 36%, and high n=12/42, 28%). Patients with high C5b-9 deposits significantly differed from the 2 other groups patients and were characterized by a significant higher histological chronicity score (p=0.005) and lower outcome-free survival (p=0.001). In multivariable analysis, higher glomerular C5b-9 remained associated with poor kidney prognosis after adjustment. One third of the 847 studied immune genes were upregulated in C3 glomerulopathy biopsies compared to controls. Unsupervised clustering on differe
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Mashup Score: 13Reduced Nephron Endowment in Six2-TGCtg Mice Is Due to Six3 ... : Journal of the American Society of Nephrology - 1 day(s) ago
long kidney function relies on the complement of nephrons generated during mammalian development from a mesenchymal nephron progenitor cell population. Low nephron endowment confers increased susceptibility to CKD. Reduced nephron numbers in the popular Six2TGC transgenic mouse line may be due to disruption of a regulatory gene at the integration site and/or ectopic expression of a gene(s) contained within the transgene. Methods Targeted locus amplification was performed to identify the integration site of the Six2TGC transgene. Genome-wide chromatin conformation capture (Hi-C) datasets were generated from nephron progenitor cells isolated from the Six2TGC+/tg mice, the Cited1CreERT2/+ control mice, and the Six2TGC+/tg; Tsc1+/Flox mice that exhibited restored nephron number compared with Six2TGC+/tg mice. Modified transgenic mice lacking the C-terminal domain of Six3 were used to evaluate the mechanism of nephron number reduction in the Six2TGC+/tg mouse line. Results Targeted locus am
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Lifelong kidney function relies on the complement of nephrons generated during mammalian development from a mesenchymal nephron progenitor cell population. These study shows the utility of Hi-C data in mapping transgene integration sites and architecture https://t.co/Keps08d2T1 https://t.co/CuryOaPuU3
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Mashup Score: 68Kidney Endothelial Cell Biology in Health and Disease : Journal of the American Society of Nephrology - 2 day(s) ago
An abstract is unavailable.
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"...the insights gained from single-cell sequencing technologies have the potential to revolutionize our understanding of the diverse populations of kidney endothelial cells..." This Editorial discusses insights into lymphatic biology for kidney diseases https://t.co/gBAku9KI7R https://t.co/CP7I1aE2Ib
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Mashup Score: 18Single-Cell RNA Sequencing Identifies Response of Renal... : Journal of the American Society of Nephrology - 2 day(s) ago
se renal lymphatic endothelial cells in quiescent and cisplatin-injured kidneys. Lymphatic endothelial cell gene expression changes were confirmed in ischemia–reperfusion injury and in cultured lymphatic endothelial cells, validating renal lymphatic endothelial cells single-cell RNA sequencing data. This study is the first to describe renal lymphatic endothelial cell heterogeneity and uncovers molecular pathways demonstrating lymphatic endothelial cells regulate the local immune response to AKI. These findings provide insights into previously unidentified molecular pathways for lymphatic endothelial cells and roles that may serve as potential therapeutic targets in limiting the progression of AKI. Background The inflammatory response to AKI likely dictates future kidney health. Lymphatic vessels are responsible for maintaining tissue homeostasis through transport and immunomodulatory roles. Owing to the relative sparsity of lymphatic endothelial cells in the kidney, past sequencing eff
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How lymphatics respond to AKI may affect AKI outcomes. This study found that lymphatic endothelial cell gene expression is altered between injury models, suggesting lymphatic endothelial cells respond to AKI may be key in regulating disease progression https://t.co/Ti2LSQUVpp https://t.co/taUltNS5kQ
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Mashup Score: 78Update on HD-Induced Multiorgan Ischemia: Brains and Beyond : Journal of the American Society of Nephrology - 4 day(s) ago
ion (IDH), abnormal perfusion to critical organs (heart, brain, liver, and kidney) and damage to vulnerable vascular beds. Recurrent conventional HD exposes patients to multiple episodes of circulatory stress, exacerbating and being aggravated by microvascular endothelial dysfunction. This promulgates progressive injury that leads to irreversible multiorgan injury and the well documented increased incidence of CV disease and premature death. This review aims to examine the underlying pathophysiology of HD-related vascular injury and to consider a range of therapeutic approach to improving outcomes set within this evolved rubric. Hemodialysis (HD) is a life-saving treatment for patients with kidney failure. However, patients requiring HD have a 10-20 times higher risk of cardiovascular (CV) morbidity and mortality than that of the general population. Patients encounter complications such as episodic intradialytic hypotension (IDH), abnormal perfusion to critical organs (heart, brain, li
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Mashup Score: 47
An abstract is unavailable.
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The updated 2019 KDOQI guidelines recognize the need to customize vascular access management in patients with advanced CKD or kidney failure. This Perspective recommends a few concrete changes in the vascular access strategy https://t.co/UEmgAJhk4v @MikeAllonK360 @DrTimmyLee1 https://t.co/uqZcKgPCjy
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Mashup Score: 37Klotho: A Large Protein with Various Beneficial Functions,... : Journal of the American Society of Nephrology - 11 day(s) ago
An abstract is unavailable. This article is available as a PDF only.
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Mashup Score: 14The Carbon Footprint of Peritoneal Dialysis in Australia : Journal of the American Society of Nephrology - 12 day(s) ago
cycle analysis to quantify CO2 equivalent emissions associated with the delivery of Baxter home automated peritoneal dialysis (APD) and continuous ambulatory peritoneal dialysis (CAPD) in Australia. Results: Annual per patient carbon emissions attributable to the manufacture and disposal of PD fluids and consumables were 1,992 kg CO2equivalent emissions for APD and 1,245 kg CO2equivalent emissions for CAPD. Transport impacts varied depending on the distance between site of manufacture of PD fluids and consumables and the home state of the patient. As a result, the total impact of providing PD also differed by Australian state, ranging from 2,350-4,503 kg CO2 equivalent emissions for APD and from 1,455-2,716 kg CO2 equivalent emissions for CAPD. Recycling of polyvinyl chloride (PVC) could reduce emissions by up to 14 per cent for APD and 30 per cent for CAPD, depending on the distance between the site of PVC waste generation and the recycling centre. Conclusions: This study demonstrated
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Mashup Score: 24Neighborhood Racial and Ethnic Segregation and the Risk of... : Journal of the American Society of Nephrology - 12 day(s) ago
merous other health disparities. Methods: We identified 901,065 older adults (age ≥55) with kidney failure from 2003 to 2019 using the United States Renal Data System (USRDS). We quantified dementia risk across tertiles of residential neighborhood segregation score using cause-specific hazard models, adjusting for individual and neighborhood-level factors. We included an interaction term to quantify the differential effect of segregation on dementia diagnosis by race and ethnicity. Results: We identified 79,851 older adults with kidney failure diagnosed with dementia between 2003 and 2019 (median follow-up: 2.2 years). Compared to those in low-segregation neighborhoods, older adults with kidney failure in high-segregation neighborhoods had a 1.63-fold (95% confidence interval (CI):1.60-1.66) higher risk of dementia diagnosis, an association that differed by race and ethnicity (Asian: adjusted hazard ratio [aHR]=1.26, 95%CI:1.15-1.38; Black: aHR=1.66, 95%CI:1.61-1.71; Hispanic: aHR=2.05
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Mashup Score: 26cGAS Activation Accelerates the Progression of Autosomal... : Journal of the American Society of Nephrology - 15 day(s) ago
mechanistic link between the deficiency polycystin-1 and mitochondrial homeostasis and the activation of the cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS)/stimulator of the interferon genes (STING) pathway. Our data identify cGAS as an important mediator of renal cystogenesis and suggest that its inhibition may be useful to slow down the disease progression. Background Immune cells significantly contribute to the progression of autosomal dominant polycystic kidney disease (ADPKD), the most common genetic disorder of the kidney caused by the dysregulation of the Pkd1 or Pkd2 genes. However, the mechanisms triggering the immune cells recruitment and activation are undefined. Methods Immortalized murine collecting duct cell lines were used to dissect the molecular mechanism of cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) activation in the context of genotoxic stress induced by Pkd1 ablation. We used conditional Pkd1 and knockout cGas−/− g
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Immune cells significantly contribute to the progression of ADPKD. This study indicates that the activation of the cGAS/STING pathway contributes to ADPKD cystogenesis through the control of the immune response associated with the loss of Pkd1 https://t.co/n3NfkQDawD @azeloglu https://t.co/Ct3vIhpaXu
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C3 glomerulopathy is a rare disease resulting from an overactivation of the complement alternative pathway. This study found intra-renal terminal pathway activation was associated with specific histological phenotype & disease prognosis in this population https://t.co/oMPM8NC6mY https://t.co/HKiUmOe7NT