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Mashup Score: 130Batch correction methods used in single cell RNA-sequencing analyses are often poorly calibrated - 1 month(s) ago
As the number of experiments that employ single-cell RNA-sequencing (scRNA-seq) grows it opens up the possibility of combining results across experiments or processing cells from the same experiment assayed in separate sequencing runs. The gain in the number of cells that can be compared comes at the cost of batch effects that may be present. Several methods have been proposed to combat this for scRNA-seq datasets. We compared seven widely used method used for batch correction of scRNA-seq datasets. We present a novel approach to measure the degree to which the methods alter the data in the process of batch correction, both at the fine scale comparing distances between cells as well as measuring effects observed across clusters of cells. We demonstrate that many of the published method are poorly calibrated in the sense that the process of correction creates measurable artifacts in the data. In particular, MNN, SCVI and LIGER performed poorly in our tests, often altering the data consi
Source: www.biorxiv.orgCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 5Persistent immune responses in the heart determine the outcome of cardiomyopathy syndrome in Atlantic salmon (Salmo salar) - 3 month(s) ago
Cardiomyopathy syndrome (CMS) caused by piscine myocarditis virus (PMCV) is a severe cardiac disease in Atlantic salmon (Salmo salar) and one of the l…
Source: www.sciencedirect.comCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 1Conference Q&A - 5 month(s) agoSource: www.youtube.comCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 3
Nature – Blood plasma protein data was combined with machine learning models for a simple method to determine differences in organ-specific aging; the study provides a basis for the prediction of…
Source: www.nature.comCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 10Despite sexual harassment shadow, biologist David Sabatini lands job at top Czech institute - 6 month(s) ago
Institute director says, “He has been punished enough,” but Czech scientists are divided
Source: www.science.orgCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 16LAG-3 as the third checkpoint inhibitor - 9 month(s) ago
Nature Immunology – LAG-3 is a T cell inhibitory receptor with a lot of promise as a target for immunotherapy, but considerable research will be needed to fully understand the nuances of this…
Source: www.nature.comCategories: Cardiologists, Latest HeadlinesTweet
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Mashup Score: 0Integrative Single-Cell Analysis of Cardiac and Pulmonary Sarcoidosis Using Publicly Available Cardiac and Bronchoalveolar Lavage Fluid Sequencing Datasets - 10 month(s) ago
Cardiac presentation of autoimmune sarcoidosis, known as cardiac sarcoidosis (CS), is a poorly understood disease with high mortality and low diagnosis rate. While CS is an immunological syndrome, little is known about how cardiac parenchymal and stromal cells mediate its pathogenesis. Moreover, while most current sarcoidosis research is based on research in pulmonary sarcoidosis (PS), it remains…
Source: FrontiersCategories: Cardiologists, Latest HeadlinesTweet
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Mashup Score: 0
President of Ukraine speaks stirringly about the indispensable ideals of freedom, self-determination, and democracy, and his nation’s fight to defend those ideals in the face of foreign aggression
Source: The HubCategories: Cardiologists, Latest HeadlinesTweet
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Mashup Score: 0Stunning of neutrophils accounts for the anti-inflammatory effects of clodronate liposomes - 12 month(s) ago
The authors show that the anti-inflammatory effects of clodronate liposomes do not result from the depletion of mononuclear phagocytes but from a functional stu
Source: Rockefeller University PressCategories: Cardiologists, Latest HeadlinesTweet
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Mashup Score: 3Salt sensitivity includes effects on immune cell signalling and metabolism - Nature Reviews Immunology - 1 year(s) ago
In this Comment article, the authors argue that recent reports showing effects of sodium on cellular immunometabolism support an expansion of the definition of salt sensitivity beyond effects on blood pressure.
Source: NatureCategories: Cardiologists, Latest HeadlinesTweet
RT @pmelsted: In this preprint https://t.co/IYUCt8DScv with @sindri_e we compared seven widely used methods for batch correction of single…