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Mashup Score: 0Dynamic responses to rejection in the transplanted human heart revealed through spatial transcriptomics - 2 day(s) ago
Allograft rejection following solid-organ transplantation is a major cause of graft dysfunction and mortality. Current approaches to diagnosis rely on histology, which exhibits wide diagnostic variability and lacks access to molecular phenotypes that may stratify therapeutic response. Here, we leverage image-based spatial transcriptomics at sub-cellular resolution in longitudinal human cardiac biopsies to characterize transcriptional heterogeneity in 62 adult and pediatric heart transplant (HT) recipients during and following histologically-diagnosed rejection. Across 28 cell types, we identified significant differences in abundance in CD4+ and CD8+ T cells, fibroblasts, and endothelial cells across different biological classes of rejection (cellular, mixed, antibody-mediated). We observed a broad overlap in cellular transcriptional states across histologic rejection severity and biological class and significant heterogeneity within rejection severity grades that would qualify for immu
Source: www.biorxiv.orgCategories: General Medicine News, Hem/OncsTweet
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Mashup Score: 0Dynamic responses to rejection in the transplanted human heart revealed through spatial transcriptomics - 5 day(s) ago
Allograft rejection following solid-organ transplantation is a major cause of graft dysfunction and mortality. Current approaches to diagnosis rely on histology, which exhibits wide diagnostic variability and lacks access to molecular phenotypes that may stratify therapeutic response. Here, we leverage image-based spatial transcriptomics at sub-cellular resolution in longitudinal human cardiac biopsies to characterize transcriptional heterogeneity in 62 adult and pediatric heart transplant (HT) recipients during and following histologically-diagnosed rejection. Across 28 cell types, we identified significant differences in abundance in CD4+ and CD8+ T cells, fibroblasts, and endothelial cells across different biological classes of rejection (cellular, mixed, antibody-mediated). We observed a broad overlap in cellular transcriptional states across histologic rejection severity and biological class and significant heterogeneity within rejection severity grades that would qualify for immu
Source: www.biorxiv.orgCategories: General Medicine News, Hem/OncsTweet-
Check out our new work! https://t.co/Qfp684iJlQ
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Mashup Score: 2Biochemistry | bioRxiv - 5 day(s) ago
bioRxiv – the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
Source: www.biorxiv.orgCategories: General Medicine News, General HCPsTweet-
New papers posted on August 13, 2024: 9 in biochemistry: https://t.co/4CFfZtfFY0 2 in bioinformatics: https://t.co/dNiMwyEYbJ 1 in biophysics: https://t.co/WExV4x2xPK 5 in cancer biology: https://t.co/7DXtDVhlLd 14 in cell biology: https://t.co/vO1EPg76bW
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Mashup Score: 0Cell Biology | bioRxiv - 5 day(s) ago
bioRxiv – the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
Source: www.biorxiv.orgCategories: General Medicine News, General HCPsTweet-
New papers posted on August 15, 2024: 10 in cell biology: https://t.co/vO1EPg76bW 18 in ecology: https://t.co/G27yHyrNIx 1 in evolutionary biology: https://t.co/0k6EOYGNAt 2 in genomics: https://t.co/Z37SNmzQpX 5 in immunology: https://t.co/I7DRZezf0S
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Mashup Score: 3Pathology | bioRxiv - 5 day(s) ago
bioRxiv – the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
Source: www.biorxiv.orgCategories: General Medicine News, General HCPsTweet-
New papers posted on September 6, 2024: 1 in pathology: https://t.co/rmmYikZh00 4 in physiology: https://t.co/fUCeEWiiwP 4 in plant biology: https://t.co/rbZpRa3Xcg 2 in systems biology: https://t.co/bu5SvDBxLG 1 in zoology: https://t.co/RLzIlMVkMR
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Mashup Score: 2Genomics | bioRxiv - 5 day(s) ago
bioRxiv – the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
Source: www.biorxiv.orgCategories: General Medicine News, General Journals & SocietTweet-
New papers posted on August 16, 2024: 13 in genomics: https://t.co/Z37SNmzQpX 6 in immunology: https://t.co/I7DRZezf0S 11 in microbiology: https://t.co/uVMmwwTmD4 4 in molecular biology: https://t.co/CnFa1ZO18j 25 in neuroscience: https://t.co/ES0ayAHKiZ
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Mashup Score: 3Developmental Biology | bioRxiv - 17 day(s) ago
bioRxiv – the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
Source: www.biorxiv.orgCategories: General Medicine News, General HCPsTweet-
New papers posted on August 13, 2024: 5 in developmental biology: https://t.co/eZVOXSRa3m 2 in ecology: https://t.co/G27yHyrNIx 2 in evolutionary biology: https://t.co/0k6EOYGNAt 1 in genetics: https://t.co/dzLSXauFuh 1 in immunology: https://t.co/I7DRZezf0S
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Mashup Score: 2Biochemistry | bioRxiv - 17 day(s) ago
bioRxiv – the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
Source: www.biorxiv.orgCategories: General Medicine News, General HCPsTweet-
New papers posted on August 13, 2024: 9 in biochemistry: https://t.co/4CFfZtfFY0 2 in bioinformatics: https://t.co/dNiMwyEYbJ 1 in biophysics: https://t.co/WExV4x2xPK 5 in cancer biology: https://t.co/7DXtDVhlLd 14 in cell biology: https://t.co/vO1EPg76bW
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Mashup Score: 0Microbiology | bioRxiv - 17 day(s) ago
bioRxiv – the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
Source: www.biorxiv.orgCategories: General Medicine News, General HCPsTweet-
New papers posted on August 13, 2024: 10 in microbiology: https://t.co/uVMmwwTmD4 4 in molecular biology: https://t.co/CnFa1ZO18j 9 in neuroscience: https://t.co/ES0ayAHKiZ 2 in plant biology: https://t.co/rbZpRa3Xcg 1 in synthetic biology: https://t.co/NvtWK7Xiza
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Mashup Score: 9
Plasmids remain by far the most common medium for delivering engineered DNA to microorganisms. However, the reliance on natural plasmid replication mechanisms limits their tunability, compatibility, and modularity. Here we refactor the natural pMB1 origin and create plasmids with customizable copy numbers by tuning refactored components. We then create compatible origins that use synthetic RNA regulators to implement independent copy control. We further demonstrate that the synthetic origin of replication (SynORI) can be engineered modularly to respond to various signals, allowing for multiplexed copy-based reporting of environmental signals. Lastly, a library of 6 orthogonal SynORI plasmids is created and co-maintained in E. coli for a week. This work establishes the feasibility of creating plasmids with SynORI that can serve as a new biotechnology for synthetic biology. ### Competing Interest Statement The authors have declared no competing interest.
Source: www.biorxiv.orgCategories: General Medicine News, Infectious DiseaseTweet-
RT @bioRxiv_synbio: Title: Engineering Plasmids with Synthetic Origins of Replication https://t.co/N4r5JrEPe2 #biorxiv_synbio
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Check out our new work! https://t.co/Qfp684iJlQ