Lyon et al. identify genetic factors important for MRSA vaginal colonization through an in vivo Tn-seq screen. Validation reveals competitive fitness defects in qoxB, bshB2, nanE, and srrAB mutants. Cross-comparison of ΔsrrAB in vitro RNA-seq with vaginal transcriptomic data uncovers >30 SrrAB-regulated factors potentially driving MRSA vaginal fitness.

Nakazawa et al. show that the cerebellar granule cell is equipped with multiple actomyosin engines to migrate in the heterogeneous microenvironment of developing brain tissue. In a 3D confined environment, PIEZO1 induces the translocation of actomyosin to the posterior membrane through PKC-ezrin signaling and generates pushing force in the migrating neuron.

Wang et al. present iMetAct, a computational method that integrates information propagation and enrichment analysis to infer metabolic enzyme activity from gene expression data. Applying iMetAct to HCC reveals three survival-associated metabolic subtypes and offers insights into the relationships between cancer cell metabolism and the surrounding immune microenvironment.