Coronary artery disease (CAD) is a common co-morbidity in people with HIV (PWH) and impaired coronary endothelial function (CEF) plays a central role in the pathogenesis of CAD. Age-related impaired CEF among PWH, however, is not well characterized. We investigated the association between CEF and age in males and females with and without HIV using 3T magnetic resonance imaging. We measured the changes in coronary cross-sectional area (CSA) and coronary blood flow during isometric handgrip exercise, an established endothelial-dependent stressor with smaller increases in CSA and coronary blood flow indicative of impaired CEF. We included 106 PWH and 82 HIV-negative individuals. Differences in demographic and clinical characteristics between PWH and HIV-negative individuals were explored using Pearson’s chi-squared test for categorical variables and Welch’s t-test for continuous variables. Linear regression models were used to examine the association between CEF and age. CEF was significa