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Mashup Score: 3Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes: STAR Protocols - 2 year(s) ago
Summary Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca2+) handling, and action potential. For complete details on the use and execution of this…
Source: star-protocols.cell.comCategories: General Medicine News, Latest HeadlinesTweet
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Mashup Score: 2
Rationale: Anthracycline-induced cardiotoxicity (AIC) is a major side effect that limits the use of anthracyclines as effective chemotherapeutics. No mechanism-based therapy is available when cardiac
Source: Circulation ResearchCategories: Cardiology News and Journals, Latest HeadlinesTweet-
Wang et al uncovered a time-dependent #autophagy dysregulation as a new mechanism for cancer patients. Learn why activation of autophagy in #cardiomyocytes is only effective in the late phase of #anthracycline-induced #cardiotoxicity at https://t.co/EPrXQXxXok @romno1 https://t.co/BYsucqpPBj
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Mashup Score: 10
Rationale: During neonatal heart regeneration, the fibrotic response, which is required to prevent cardiac rupture, resolves via poorly understood mechanisms. Deletion of the Hippo pathway gene Sav i
Source: Circulation ResearchCategories: Cardiology News and Journals, Latest HeadlinesTweet-
Liu et al found that #Wntless (Wls) is a direct target of #YAP in #cardiomyocytes and is required for neonatal heart regeneration. Learn more about how Wls signals between cardiomyocytes and fibroblasts to suppress #fibrosis https://t.co/4mWGyTVl7r @jmartinlab https://t.co/YvfvPUwxEU
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Mashup Score: 18
Rationale: Dominant heterozygous variants in filamin C (FLNC) cause diverse cardiomyopathies, although the underlying molecular mechanisms remain poorly understood. Objective: We aimed to define the
Source: Circulation ResearchCategories: Cardiology News and Journals, Latest HeadlinesTweet-
Agarwal & colleagues found altered #protein turnover and increased #lysosome content in human #iPSC derived #cardiomyocytes with #FLNC variants that cause #cardiomyopathy. Learn more at https://t.co/UAr6YxCghR @radhikawal @christoepfer @subra_sun https://t.co/W4OAINZtXF
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Mashup Score: 14
Abstract. Manifestations of cardiovascular diseases (CVDs) in a patient or a population differ based on inherent biological makeup, lifestyle, and exposure to e
Source: OUP AcademicCategories: Cardiology News and Journals, Latest HeadlinesTweet-
Getting up to speed on human-induced pluripotent #stemcells State-of-the-art review highlights the translational benefits of #iPSC-derived #cardiomyocytes to model disease & test therapies in #CardiovascularResearch https://t.co/3XmLIUKnwD @CVR_TomaszGuzik @glenpyle @Joseph_C_Wu https://t.co/Wf814TdOmV
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Mashup Score: 0Use of Proximity Labeling in Cardiovascular Research: - 3 year(s) ago
Highlights • Protein interaction networks are of critical importance in regulating cardiac physiology, including adrenergic signaling. • The proximity labeling approach is based on the enzymatic-de…
Categories: Cardiac Surgery, Latest HeadlinesTweet-
How can proximity labeling reveal novel protein-protein interactions important in cardiac physiology? Learn more in this state-of-the-art review in this issue of #JACCBTS: https://t.co/7JLic668AL #OpenAccess #cvResearch #MedTwitter #EKG #cardiomyocytes #CVD https://t.co/e4vtUMRcAP
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Mashup Score: 0
Rationale: Atrial fibrillation (AF) is the most common cardiac arrhythmia diagnosed in clinical practice. Genome-wide association studies have identified AF-associated common variants across 100+ gen
Source: Circulation ResearchCategories: Cardiology News and Journals, Latest HeadlinesTweet-
Bosada & colleagues found in #cardiomyocytes a variant non-coding region that controls expression of #Prrx1 and reduction causes altered cardiac #electrophysiology and predisposes the heart to #AFib. Learn more at https://t.co/9pjawcEfxJ @Vince88888 @amsterdamumc https://t.co/A59MYfESj4
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Mashup Score: 1
Rationale: Reperfusion injury accounts for up to half of myocardial infarct size, and meaningful clinical therapies targeting it do not exist. We have reported previously that autophagy is reduced du
Source: Circulation ResearchCategories: Cardiology News and Journals, Latest HeadlinesTweet-
Xie et al found that #Beclin protects #cardiomyocytes by inducing autophagic flux and decreasing #ROS following cardiac #IschemiaReperfusion injury. Learn more about how inducing #autophagy at reperfusion is cardioprotective https://t.co/YsvtB4EYsR @josephahill https://t.co/xLIa4tvJT8
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Mashup Score: 0
Rationale: Pyroptosis is a morphologically and mechanistically distinct form of cell death and is characterized by GSDMD (gasdermin D) or GSDME (gasdermin E)-mediated necrosis with excessive inflamma
Source: Circulation ResearchCategories: Cardiology News and Journals, Latest HeadlinesTweet-
Shi & colleagues found #OxidativeStress activated #caspase-11 & #GSDMD in #cardiomyocytes, caused #pyroptosis as well as aggravated myocardial #IschemiaReperfusion injury. Learn more at https://t.co/kKOG1hqSBC @FudanUniv https://t.co/TGXumClTCP
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Mashup Score: 3Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes: STAR Protocols - 3 year(s) ago
Summary Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). Here, we describe a cost-effective strategy for the subsequent massive expansion (>250-fold) of high-purity hiPSC-CMs relying on two aspects: removal of cell-cell contacts and small-molecule…
Source: star-protocols.cell.comCategories: General Medicine News, Latest HeadlinesTweet-
Check out this #protocol for massive expansion and #cryopreservation of functional #hiPSC-derived #cardiomyocytes, from Dr. Jan W. Buikema and @SeanM_Wu @Stanford @STARProtocols #ASGCT21 https://t.co/hMaeV9oNpw https://t.co/y8LrfKAhIR
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Online in @STARProtocols: Dr. Joseph C. Wu @Joseph_C_Wu @StanfordCVI describing the measurement of contraction, #CalciumImaging, and imaging-based #ActionPotential measurement in #hiPSC-derived #cardiomyocytes: https://t.co/ixk04ULGt3 https://t.co/W45FVxURKS