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Mashup Score: 9
Background Hematopoietic acute radiation syndrome (H-ARS) occurring after exposure to ionizing radiation damages bone marrow causing cytopenias, increasing susceptibility to infections and death. We and others have shown that cellular therapies like human mesenchymal stromal cells (MSCs), or monocytes/macrophages educated ex-vivo with extracellular vesicles (EVs) from MSCs were effective in a lethal H-ARS mouse model. However, given the complexity of generating cellular therapies and the potential risks of using allogeneic products, development of an “off-the-shelf” cell-free alternative like EVs may have utility in conditions like H-ARS that require rapid deployment of available therapeutics. The purpose of this study was to determine the feasibility of producing MSC-derived EVs at large scale using a bioreactor and assess critical quality control attributes like identity, sterility, and potency in educating monocytes and promoting survival in a lethal H-ARS mouse model. Methods EVs w
Source: stemcellres.biomedcentral.comCategories: General Medicine News, Hem/OncsTweet
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Mashup Score: 0Rheumatoid arthritis: the old issue, the new therapeutic approach - Stem Cell Research & Therapy - 7 month(s) ago
Rheumatoid arthritis (RA) is a chronic and systemic autoimmune disease of unknown etiology. The most common form of this disease is chronic inflammatory arthritis, which begins with inflammation of the synovial membrane of the affected joints and eventually leads to disability of the affected limb. Despite significant advances in RA pharmaceutical therapies and the availability of a variety of medicines on the market, none of the available medicinal therapies has been able to completely cure the disease. In addition, a significant percentage (30–40%) of patients do not respond appropriately to any of the available medicines. Recently, mesenchymal stromal cells (MSCs) have shown promising results in controlling inflammatory and autoimmune diseases, including RA. Experimental studies and clinical trials have demonstrated the high power of MSCs in modulating the immune system. In this article, we first examine the mechanism of RA disease, the role of cytokines and existing medicinal thera
Source: stemcellres.biomedcentral.comCategories: General Medicine Journals and Societies, Latest HeadlinesTweet-
A Review in Stem Cell Research & Therapy examines the mechanism of rheumatoid arthritis (RA), the role of cytokines and existing therapies, and the immunomodulatory function of mesenchymal stromal cells in the treatment of RA and other autoimmune diseases. https://t.co/DYZvLP9hlb
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Mashup Score: 1Targeted screening and identification of chlorhexidine as a pro-myogenic circadian clock activator - Stem Cell Research & Therapy - 9 month(s) ago
Background The circadian clock is an evolutionarily conserved mechanism that exerts pervasive temporal control in stem cell behavior. This time-keeping machinery is required for orchestrating myogenic progenitor properties in regenerative myogenesis that ameliorates muscular dystrophy. Here we report a screening platform to discover circadian clock modulators that promote myogenesis and identify chlorhexidine (CHX) as a clock-activating molecule with pro-myogenic activities. Methods A high-throughput molecular docking pipeline was applied to identify compounds with a structural fit for a hydrophobic pocket within the key circadian transcription factor protein, Circadian Locomotor Output Cycles Kaput (CLOCK). These identified molecules were further screened for clock-modulatory activities and functional validations for pro-myogenic properties. Results CHX was identified as a clock activator that promotes distinct aspects of myogenesis. CHX activated circadian clock that reduced cycling
Source: stemcellres.biomedcentral.comCategories: Healthcare Professionals, Latest HeadlinesTweet-
Targeted screening and identification of chlorhexidine as a pro-myogenic circadian clock activator | Stem Cell Research & Therapy | Full Text https://t.co/Myflyim0zZ
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Congrats to John Kink and team @WiscPediatrics @uwsmph @UWCarbone @UWSCRMC @MCWCancerCenter for developing a GMP compliant protocol for generating #exosomes for hematopoietic acute radiation syndrome https://t.co/0oa9adDY4o