Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence
Cappabianca and colleagues manufactured virus-free CAR T cells at scale with CRISPR/Cas9 and ‘metabolically primed’ by attenuating activation in low glucose/glutamine media with expansion in high glucose/glutamine media. Priming made CAR T-cells with increased stem cell memory properties, including enriched central memory phenotypes in vivo while lysing solid tumors.