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Mashup Score: 37
Current technologies for upregulation of endogenous genes use targeted artificial transcriptional activators but stable gene activation requires persistent expression of these synthetic factors. Although general “hit-and-run” strategies exist for inducing long-term silencing of endogenous genes using targeted artificial transcriptional repressors, to our knowledge no equivalent approach for gene activation has been described to date. Here we show stable gene activation can be achieved by harnessing endogenous transcription factors ( EndoTF s) that are normally expressed in human cells. Specifically, EndoTFs can be recruited to activate endogenous human genes of interest by using CRISPR-based gene editing to introduce EndoTF DNA binding motifs into a target gene promoter. This Precision Editing of Regulatory Sequences to Induce Stable Transcription-On ( PERSIST-On ) approach results in stable long-term gene activation, which we show is durable for at least five months. Using a high-thro
Source: www.biorxiv.orgCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 37
Current technologies for upregulation of endogenous genes use targeted artificial transcriptional activators but stable gene activation requires persistent expression of these synthetic factors. Although general “hit-and-run” strategies exist for inducing long-term silencing of endogenous genes using targeted artificial transcriptional repressors, to our knowledge no equivalent approach for gene activation has been described to date. Here we show stable gene activation can be achieved by harnessing endogenous transcription factors ( EndoTF s) that are normally expressed in human cells. Specifically, EndoTFs can be recruited to activate endogenous human genes of interest by using CRISPR-based gene editing to introduce EndoTF DNA binding motifs into a target gene promoter. This Precision Editing of Regulatory Sequences to Induce Stable Transcription-On ( PERSIST-On ) approach results in stable long-term gene activation, which we show is durable for at least five months. Using a high-thro
Source: www.biorxiv.orgCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 2
People with two copies of the gene variant APOE4 are almost certain to get Alzheimer’s, say researchers, who proposed a framework under which such patients could be diagnosed years before symptoms.
Source: www.nytimes.comCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 94In a scientific first, researchers use CRISPR base editing to treat liver disease in fetal monkeys - 17 day(s) ago
In a scientific first, researchers have used CRISPR base editing to treat liver disease in fetal monkeys.
Source: www.statnews.comCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 94In a scientific first, researchers use CRISPR base editing to treat liver disease in fetal monkeys - 17 day(s) ago
In a scientific first, researchers have used CRISPR base editing to treat liver disease in fetal monkeys.
Source: www.statnews.comCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 2Doctors Can Now Edit the Genes Inside Your Body - 2 month(s) ago
It sounds like science fiction, but dozens of people have undergone gene editing for cardiovascular disease and other conditions.
Source: www.wsj.comCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 2Doctors Can Now Edit the Genes Inside Your Body - 2 month(s) ago
It sounds like science fiction, but dozens of people have undergone gene editing for cardiovascular disease and other conditions.
Source: www.wsj.comCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 19
Tippi MacKenzie envisions doing surgery without scalpels or sutures, editing fetal genes to prevent inherited disorders.
Source: www.statnews.comCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 19
Tippi MacKenzie envisions doing surgery without scalpels or sutures, editing fetal genes to prevent inherited disorders.
Source: www.statnews.comCategories: General Medicine News, CardiologistsTweet
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Mashup Score: 19
Tippi MacKenzie envisions doing surgery without scalpels or sutures, editing fetal genes to prevent inherited disorders.
Source: www.statnews.comCategories: General Medicine News, CardiologistsTweet
RT @JKeithJoung: Excited to share a new preprint (https://t.co/nPXJf7FW19) describing Precision Editing of Regulatory Sequences to Induce S…