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Mashup Score: 12
Aims This retrospective non-randomised study aims to identify new and rare fusion partners with USP6 in the setting of nodular fasciitis. It has been proven, that nodular fasciitis can harbour different variants of USP6 fusions, which can be used in routine diagnostics and even determine the biological behaviour of the process. Methods A total of 19 cases of nodular fasciitis examined between 2011 and 2022 at Motol University Hospital in Prague were included into this study. Next to the histopathological evaluation, all cases were assessed using immunohistochemistry, RT-PCR and Anchored multiplex RNA methods. Patientโs main demographic characteristics and corresponding clinical data were also analysed. Results This study presents one novel ( KIF1A ) and five rare examples ( TMP4, SPARC, EIF5A, MIR22HG, COL1A2 ) of fusion partners with USP6 among 19 cases of nodular fasciitis. Conclusion Identification of USP6 fusion partners in nodular fasciitis helps to understand the biology of such
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Mashup Score: 17Risk assessment in pT1 colorectal cancer - 1 day(s) ago
Colorectal cancer (CRC) is a common malignancy worldwide and tumour stage is closely related to clinical outcome. A small but significant proportion of submucosal-invasive (ie, pT1) CRC are associated with regional lymph node metastases (LNM) and a worse prognosis. The likelihood of LNM in pT1 CRC needs to be balanced against the operative risk and costs of surgical resection when determining the best patient management. A wide range of histopathological and clinical factors may affect LNM risk in this setting. This script provides a comprehensive overview of the tumour and patient-associated features that have been linked to LNM risk in pT1 CRC. Some of the features are well established within the literature and are included in published guidelines, while others are novel and emerging in nature. Odds ratios for LNM that are associated with key predictive features are provided where appropriate, and published models developed as an aid to the calculation of LNM risk are discussed.
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Mashup Score: 12
Aims Preferentially expressed antigen in melanoma (PRAME) recently is a reliable immunohistochemistry (IHC) marker for distinguishing melanoma from other lesions. However, there are few articles focused on PRAME use in acral malignant melanoma, the most common type in Asians. This study investigated PRAME IHC expression in a large series of acral malignant melanoma in situ to add to the body of clinical knowledge. Methods PRAME IHC was performed in unequivocal cases of primary acral lentiginous melanoma in situ (ALMIS), subungual melanoma in situ (SMIS) and acral recurrent nevi as the control. PRAME tumour cell percentage positivity and intensity were expressed as categorised in a cumulative score by adding the quartile of positive tumour cells to intensity labelling. The final IHC expression was interpreted as negative (0โ1), weak (2โ3), moderate (4โ5) or strong (6โ7). Results In 91 ALMIS patients, 32 cases (35.16%) were strong, 37 (40.66%) were moderate and 22 (24.18%) were weak. In
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Mashup Score: 17Risk assessment in pT1 colorectal cancer - 4 day(s) ago
Colorectal cancer (CRC) is a common malignancy worldwide and tumour stage is closely related to clinical outcome. A small but significant proportion of submucosal-invasive (ie, pT1) CRC are associated with regional lymph node metastases (LNM) and a worse prognosis. The likelihood of LNM in pT1 CRC needs to be balanced against the operative risk and costs of surgical resection when determining the best patient management. A wide range of histopathological and clinical factors may affect LNM risk in this setting. This script provides a comprehensive overview of the tumour and patient-associated features that have been linked to LNM risk in pT1 CRC. Some of the features are well established within the literature and are included in published guidelines, while others are novel and emerging in nature. Odds ratios for LNM that are associated with key predictive features are provided where appropriate, and published models developed as an aid to the calculation of LNM risk are discussed.
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Mashup Score: 15Enteroblastic gastric cancer subtype holds therapeutic clues - 6 day(s) ago
In this issue of the Journal of Clinical Pathology , Pu and colleagues from the Nanjing University Medical School, China, report that NTRK gene alterations were enriched in gastric carcinoma with hepatoid or enteroblastic differentiation but not, as in the colon, dMMR-type gastric carcinomas.1 The study analysed 51 cases of EBV (Epstein-Barr virus)-associated gastric carcinomas, 94 cases of dMMR gastric carcinomas, 90 cases of gastric adenocarcinoma with hepatoid or enteroblastic differentiation and 256 cases of conventional gastric carcinomas. All four tumours with NTRK gene alterations were identified in gastric carcinoma with hepatoid or enteroblastic differentiation. Among these four cases, two were associated with fusion ( NTRK2-SMCHD1 fusion and TPM3-NTRK1 ) and two with amplification ( NTRK1 and NTRK3 ). The role of NTRK amplification is unclear, but emerging evidence suggests that these genetic alterations may also be targetable.2 The study adds to the growing literature that g
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All you need to know about hepatoid carcinomas. An editorial and a new study uncover NTRK alterations in hepatoid carcinomas and adenocarcinomas with enteroblastic differentiation. ๐๐ฌ Don't miss our latest insights! ๐๐งฌhttps://t.co/eqs9qfhvd1 https://t.co/C8C24d60FI https://t.co/H1GFnsQAam
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Mashup Score: 11Emerging and under-recognised patterns of colorectal carcinoma morphologies: a comprehensive review - 7 day(s) ago
While the overwhelming majority of colorectal carcinomas (CRC) are diagnosed as adenocarcinoma not otherwise specified, there are numerous under-recognised morphologic patterns of CRC. These patterns are recognised by the WHO, appear in reporting manuals for the American Joint Committee of Cancer, and/or are listed on synoptic reports, while many other variants have either fallen out of favour or are emerging as future bona fide patterns. Herein, we discuss 13 variants: serrated adenocarcinoma, micropapillary adenocarcinoma, medullary carcinoma, neuroendocrine carcinoma, mucinous adenocarcinoma, signet-ring cell carcinoma, adenosquamous carcinoma, adenoma-like adenocarcinoma, lymphoglandular complex-like CRC, carcinoma with sarcomatoid components, cribriform-comedo-type adenocarcinoma, undifferentiated carcinoma and low-grade tubuloglandular adenocarcinoma. The purpose of this review is to scrutinise these variants by assessing their clinical characteristics, morphologic cues, as well
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How well do you know your variants of colon cancer? ๐ค๐ฌ The WHO ๐ has its list of recognized variants, but do you know which ones are important, which are no longer recognized, and which have been discarded? ๐ซ๐ก Dive into the details and stay informed! https://t.co/WIZWh0cJeR https://t.co/M7RuSillqK
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Mashup Score: 10
Aims Pre-surgical risk classification tools for prostate cancer have shown better patient stratification with the addition of cribriform pattern 4 (CC) and intraductal prostatic carcinoma (IDC) identified in biopsies. Here, we analyse the additional prognostic impact of CC/IDC observed in prostatectomies using Cancer of Prostate Risk Assessment post-surgical (CAPRA-S) stratification. Methods A retrospective cohort of treatment-naรฏve radical prostatectomy specimens from three North American academic institutions (2010โ2018) was assessed for the presence of CC/IDC. Patients were classified, after calculating the CAPRA-S scores, into low-risk (0โ2), intermediate-risk (3โ5) and high-risk (6โ12) groups. Kaplan-Meier curves were created to estimate biochemical recurrence (BCR)-free survival. Prognostic performance was examined using Harrellโs concordance index, and the effects of CC/IDC within each risk group were evaluated using the Cox proportional hazards models. Results Our cohort includ
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Mashup Score: 22Spatial resolution of renal amyloid deposits through MALDI-MSI: a combined digital and molecular approach to monoclonal gammopathies - 9 day(s) ago
Aims Identification and characterisation of monoclonal gammopathies of renal significance (MGRS) is critical for therapeutic purposes. Amyloidosis represents one of the most common forms of MGRS, and renal biopsy remains the gold standard for their classification, although mass spectrometry has shown greater sensitivity in this area. Methods In the present study, a new in situ proteomic technique, matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI), is investigated as an alternative to conventional laser capture microdissection MS for the characterisation of amyloids. MALDI-MSI was performed on 16 cases (3 lambda light chain amyloidosis (AL), 3 AL kappa, 3 serum amyloid A amyloidosis (SAA), 2 lambda light chain deposition disease (LCDD), 2 challenging amyloid cases and 3 controls). Analysis began with regions of interest labelled by the pathologist, and then automatic segmentation was performed. Results MALDI-MSI correctly identified and typed cases with k
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โถ๏ธ MALDI-MSI renal amyloidosis โถ๏ธ 16 cases amyloidosis โถ๏ธ high sensitivity /specificity in identifying and typing known amyloid 'โถ๏ธ restricted fingerprint' for amyloid detection composed of apolipoprotein E, serum amyloid protein, and apolipoprotein A1 https://t.co/WTPb1aIbSB https://t.co/3NCAqEbEHQ
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Mashup Score: 2Best practice for LDL-cholesterol: when and how to calculate - 9 day(s) ago
The lipid profile is important in the risk assessment for cardiovascular disease. The lipid profile includes total cholesterol, high-density lipoprotein (HDL)-cholesterol, triglycerides (TGs) and low-density lipoprotein (LDL)-cholesterol (LDL-C). LDL-C has traditionally been calculated using the Friedewald equation (invalid with TGs greater than 4.5โmmol/L and is based on the assumption that the ratio of TG to cholesterol in very- low-density lipoprotein (VLDL) is 5 when measured in mg /dL). LDL-C can be quantified with a reference method, beta-quantification involving ultracentrifugation and this is unsuitable for routine use. Direct measurement of LDL-C was expected to provide a solution with high TGs. However, this has some challenges because of a lack of standardisation between the reagents and assays from different manufacturers as well as the additional costs. Furthermore, mild hypertriglyceridaemia also distorts direct LDL-C measurements. With the limitations of the Friedewald e
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Mashup Score: 11Emerging and under-recognised patterns of colorectal carcinoma morphologies: a comprehensive review - 11 day(s) ago
While the overwhelming majority of colorectal carcinomas (CRC) are diagnosed as adenocarcinoma not otherwise specified, there are numerous under-recognised morphologic patterns of CRC. These patterns are recognised by the WHO, appear in reporting manuals for the American Joint Committee of Cancer, and/or are listed on synoptic reports, while many other variants have either fallen out of favour or are emerging as future bona fide patterns. Herein, we discuss 13 variants: serrated adenocarcinoma, micropapillary adenocarcinoma, medullary carcinoma, neuroendocrine carcinoma, mucinous adenocarcinoma, signet-ring cell carcinoma, adenosquamous carcinoma, adenoma-like adenocarcinoma, lymphoglandular complex-like CRC, carcinoma with sarcomatoid components, cribriform-comedo-type adenocarcinoma, undifferentiated carcinoma and low-grade tubuloglandular adenocarcinoma. The purpose of this review is to scrutinise these variants by assessing their clinical characteristics, morphologic cues, as well
Source: jcp.bmj.comCategories: General Medicine News, General Journals & SocietTweet-
How well do you know your variants of colon cancer? ๐ค๐ฌ The WHO ๐ has its list of recognized variants, but do you know which ones are important, which are no longer recognized, and which have been discarded? ๐ซ๐ก Dive into the details and stay informed! https://t.co/WIZWh0cJeR https://t.co/M7RuSillqK
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๐ Novel fusions in nodular fasciitis ๐ 13 of 19 (68%) MYH9::USP6 fusion โถ๏ธ 6 unusual USP6 fusion partners TPM4, EIF5A, SPARC, MIR22HG, COL1A2 and KIF1A. https://t.co/6b2vfT0WFA https://t.co/XHS3Vk7gYh