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    You are invited to join ASM Editors in Chief for a round table discussion about ASM’s Open Access titles. Live Q&A to follow. Audience participation is highly encouraged! Featuring. Dr. Irene Newton (MRA), Dr. Cristina Cuomo (Microbiology Spectrum), Dr. Mike Imperiale (mSphere), Dr. Jack Gilbert…

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    Summary Gnotobiology has revolutionized the study of microbiota-host interactions. This protocol explains how to generate, maintain, and monitor gnotobiotic mice. Three monitoring methods are presented and compared: bacterial culture, microscopy to visualize the presence (or absence) of bacteria using Gram staining or DNA staining, and 16S rRNA gene amplification and sequencing. The generation…

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