Clostridioides difficile (C. difficile) is a gram-positive, toxin-forming, anaerobic bacillus, which is widely distributed in the intestinal tract of living organisms.1–3 The pathogenic properties are produced by toxins released into the gastrointestinal tract, in conjunction with a loss of balance within the gastrointestinal flora.4 C. difficile produces 2 protein exotoxins, toxin A and toxin B, which cause colonic mucosal injury. These toxins are not only responsible for active infection but are also targeted for testing purposes of C.