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Mashup Score: 1Deficiency of MicroRNA-181a Results in Transcriptome-Wide Cell-Specific Changes in the Kidney and Increases Blood Pressure - 3 year(s) ago
MicroRNA miR-181a is downregulated in the kidneys of hypertensive patients and hypertensive mice. In vitro, miR-181a is a posttranslational inhibitor of renin expression, but pleiotropic mechanisms b
Source: HypertensionCategories: Cardiology News and Journals, Latest HeadlinesTweet
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Mashup Score: 5Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library: STAR Protocols - 3 year(s) ago
Summary The development of CRISPR-Cas9 screening techniques coupled with chemical inhibition of specific biological processes enables high-throughput investigation into many areas of molecular biology. We present a protocol to conduct ubiquitin proteasome system-specific chemical-genetic CRISPR-Cas9 screens in the human HAP1 cell line. This protocol can be adapted for use…
Source: star-protocols.cell.comCategories: General Medicine News, Latest HeadlinesTweet-
Online in @STARProtocols: Dr. David Toczyski @Ucsf_Biochem, describes chemical-genetic #CRISPR_Cas9 screens in human cells using a pathway-specific library https://t.co/1drvHM9FVR https://t.co/0Y1b8qXy3q
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Mashup Score: 2Generating mutant Aedes aegypti mosquitoes using the CRISPR/Cas9 system: STAR Protocols - 3 year(s) ago
Summary Implementation of CRISPR/Cas9 methodologies for mosquito gene editing has not yet become widespread. This protocol details the procedure for Aedes aegypti mosquito gene editing using homology-directed repair and fluorescent marker insertion, which facilitates the generation and screening of mutant mosquito lines for gene function testing. We describe optimized methods for single guide RNA…
Source: star-protocols.cell.comCategories: General Medicine News, Latest HeadlinesTweet-
Online in @STARProtocols: #CRISPR_Cas9 #protocol for Aedes aegypti #mosquito gene editing by Dr. Chun-Hong Chen @CHUNHONGCHEN4, using homology-directed repair and fluorescent marker insertion for generation and screening of mutant mosquito lines https://t.co/n5Weev5GSO https://t.co/DI642uAxcU
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This study led by @FZMarques and @GeoffreyHead combines a new #CRISPR_Cas9 model #RNAScope @ACDbio and #scRNA_seq technology with traditional techniques such as telemetry and #renin #IHC to describe how #miR181a regulates #BloodPressure #MicroRNA #SSBP. https://t.co/AmjN6duS2C https://t.co/jmCqTDgiVI