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Mashup Score: 7Collagen type XII is undetectable in keratoconus Bowman’s layer - 2 month(s) ago
Purpose Corneal biomechanical failure is the hallmark of keratoconus (KC); however, the cause of this failure remains elusive. Collagen type XII ( COL12A1 ), which localises to Bowman’s layer (BL), is thought to function in stress-bearing areas, such as BL. Given the putative protective role of COL12A1 in biomechanical stability, this study aims to characterise COL12A1 expression in all corneal layers involved in KC. Methods TaqMan quantitative PCR was performed on 31 corneal epithelium samples of progressive KC and myopic control eyes. Tissue microarrays were constructed using full-thickness corneas from 61 KC cases during keratoplasty and 18 non-KC autopsy eyes and stained with an antibody specific to COL12A1. Additionally, COL12A1 was knocked out in vitro in immortalised HEK293 cells. Results COL12A1 expression was reduced at transcript levels in KC epithelium compared with controls (ratio: 0.58, p<0.03). Immunohistochemical studies demonstrated that COL12A1 protein expression in BL
Source: bjo.bmj.comCategories: General Medicine News, OphthalmologyTweet
What is the role of #collagen type XII (COL12A1) in the pathogenesis of #keratoconus? A new study from Rigi et al., reveals the implication that in keratoconus patients, COL12A1 in Bowman's layer is undetectable. Read here: https://t.co/HY2shKJeWu #ophthalmology #vision #cornea https://t.co/EbYmc2qVZt