Spatially exploring RNA biology in archival formalin-fixed paraffin-embedded tissues
Patho-DBiT enables spatial profiling of a range of RNA species (i.e., mRNA, microRNA, tRNA, splicing isoform, etc.) all at once in clinical formalin-fixed paraffin-embedded tissue with high sensitivity and coverage. It further allows for genome-wide mapping of single-nucleotide RNA variants to distinguish subclones and dissect spatiotemporal tumorigenesis at the cellular level.