m6A modification inhibits miRNAs’ intracellular function, favoring their extracellular export for intercellular communication
Garbo et al. report that mature miRNAs are methylated (m6A) in a cell-specific manner and that highly methylated miRNAs are impaired in AGO2 association and in their intracellular function. m6A modification enhances extracellular vesicle (EV) A2B1-mediated compartmentalization. Notably, receiving cells demethylate EV-delivered miRNAs and restore their function.