Derivation of functional thymic epithelial organoid lines from adult murine thymus
Lim et al. establish a condition to generate 3D thymic epithelial cell (TEC) organoids from the adult murine thymus. The TEC organoids allow long-term expansion and differentiation to functional mTECs and cTECs in vitro. TEC organoids successfully support T cell development in vitro and in nude mice in vivo after transplantation.