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Mashup Score: 0acCRISPR: an activity-correction method for improving the accuracy of CRISPR screens - PubMed - 2 year(s) ago
High throughput CRISPR screens are revolutionizing the way scientists unravel the genetic underpinnings of engineered and evolved phenotypes. One of the critical challenges in accurately assessing screening outcomes is accounting for the variability in sgRNA cutting efficiency. Poorly active guides …
Source: PubMedCategories: Future of Medicine, Latest HeadlinesTweet
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Mashup Score: 0
Pten and p53 are two of the most frequently mutated tumor suppressor genes in endometrial cancer. However, the functional consequences and histopathological manifestation of concomitant p53 and Pten loss of function alterations in the development of endometrial cancer is still controversial. Here, we demonstrate that simultaneous Pten and p53 deletion is sufficient to cause epithelial to…
Source: bioRxivCategories: Future of Medicine, Latest HeadlinesTweet-
In vivo intra-uterine delivery of TAT-fused Cre recombinase and CRISPR/Cas9 editing unveil histopathology of Pten/p53-deficient endometrial cancers. https://t.co/PIzF97dHSE
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Mashup Score: 0A CRISPR-Cas12a-based platform for ultrasensitive rapid highly specific detection of Mycobacterium tuberculosis in clinical application - PubMed - 2 year(s) ago
Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the second leading cause of death after COVID-19 pandemic. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based biosensing system to design a novel detection platform for tuberculosis …
Source: PubMedCategories: Future of Medicine, Latest HeadlinesTweet-
A CRISPR-Cas12a-based platform for ultrasensitive rapid highly specific detection of Mycobacterium tuberculosis in clinical application https://t.co/c1NhtPAI26
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Mashup Score: 0A systematic review of computational methods for designing efficient guides for CRISPR DNA base editor systems - PubMed - 2 year(s) ago
In only a few years, as a breakthrough technology, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) gene-editing systems have ushered in the era of genome engineering with a plethora of applications. One of the most promising CRISPR tools, so-called ba …
Source: PubMedCategories: Future of Medicine, Latest HeadlinesTweet-
A systematic review of computational methods for designing efficient guides for CRISPR DNA base editor systems https://t.co/XDrBb6Jcst
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Mashup Score: 0Molecular mechanisms of Streptococcus pyogenes Cas9: a single-molecule perspective - PubMed - 2 year(s) ago
Cas9 is an RNA-guided endonuclease from the type II CRISPR-Cas system that employs RNA-DNA base pairing to target and cleave foreign DNA in bacteria. Due to its robust and programmable activity, Cas9 has been repurposed as a revolutionary technology for wide-ranging biological and medical applicatio …
Source: PubMedCategories: Future of Medicine, Latest HeadlinesTweet-
Molecular mechanisms of Streptococcus pyogenes Cas9: a single-molecule perspective https://t.co/NMM1o7DT9D
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Mashup Score: 0
The crosstalk between prostate cancer (PCa) cells and the tumor microenvironment plays a pivotal role in disease progression and metastasis and could provide novel opportunities for patient treatment. Macrophages are the most abundant immune cells in the prostate tumor microenvironment (TME) and are capable of killing tumor cells. To identify genes in the tumor cells that are critical for…
Source: bioRxivCategories: Future of Medicine, Latest HeadlinesTweet-
A genome-wide CRISPR screen in human prostate cancer cells reveals drivers of macrophage-mediated cell killing and positions AR as a tumor-intrinsic immunomodulator https://t.co/NcgcIs7omn
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Mashup Score: 0CRISPR-Cas12a-Based Rapid and Sensitive Detection of rpoBL378R in Mycobacterium tuberculosis - 2 year(s) ago
Rifampin is the most effective drug in the treatment of tuberculosis, whose major pathogen is Mycobacterium tuberculosis (MTB), whereas there are still certain MTB strains resistant to the therapy of rifampin. The rpoB mutations play a central role in MTB resistance to the rifampin therapy, so it is crucial to identify these mutations in order to discover novel therapeutic approaches to these…
Source: bioRxivCategories: Future of Medicine, Latest HeadlinesTweet-
CRISPR-Cas12a-Based Rapid and Sensitive Detection of rpoBL378R in Mycobacterium tuberculosis https://t.co/rZfQWPawiN
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Mashup Score: 0Recent advances in stimuli-responsive polymeric carriers for controllable CRISPR/Cas9 gene editing system delivery - PubMed - 2 year(s) ago
Non-viral polymeric vectors with good biocompatibility have been recently explored as delivery systems for clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) nucleases. In this review, based on current limitations and critical barriers, we summarize the advantages of …
Source: PubMedCategories: Future of Medicine, Latest HeadlinesTweet-
Recent advances in stimuli-responsive polymeric carriers for controllable CRISPR/Cas9 gene editing system delivery https://t.co/7sjVgqvWmZ
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Mashup Score: 0Elevated nuclear PIGL disrupts the cMyc/BRD4 axis and improves PD-1 blockade therapy by dampening tumor immune evasion - PubMed - 2 year(s) ago
To improve the efficacy of lenvatinib in combination with programmed death-1 (PD-1) blockade therapy for hepatocellular carcinoma (HCC), we screened the suppressive metabolic enzymes that sensitize HCC to lenvatinib and PD-1 blockade, thus impeding HCC progression. After analysis of the CRISPR‒Cas9 …
Source: PubMedCategories: Future of Medicine, Latest HeadlinesTweet-
Elevated nuclear PIGL disrupts the cMyc/BRD4 axis and improves PD-1 blockade therapy by dampening tumor immune evasion https://t.co/W62dkvmssL
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Mashup Score: 0
Genetic code expansion via amber suppression allows cotranslational, site-specific introduction of nonnatural chemical groups into proteins in the living cell. The archaeal pyrrolysine-tRNA/pyrrolysine-tRNA synthetase (PylT/RS) pair from Methanosarcina mazei (Mma) has been established for incorporat …
Source: PubMedCategories: Future of Medicine, Latest HeadlinesTweet-
Generation of Amber Suppression Cell Lines Using CRISPR-Cas9 https://t.co/o9GAF0kRwd
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acCRISPR: an activity-correction method for improving the accuracy of CRISPR screens https://t.co/NXYvJYOu0l