an times was performed over a 5 mm × 5 mm field-of-view with 600 kHz A-scan rate. Second-generation variable interscan time analysis OCTA was used to measure a surrogate marker for capillary blood flow speed, variable interscan time analysis flow speed (VFS), in the superficial and intermediate capillary plexusesVFS, and deep capillary plexusVFS. Vessel density was measured using OCTA. Results: Fifty-seven eyes with different diabetic retinopathy severity and 37 normal eyes were analyzed. Variable interscan time analysis OCTA provided diverse blood flow speed information, including pseudo-color OCTA and mean flow speed in different regions. Both deep capillary plexusVFS and deep capillary plexusVFS/(superficial and intermediate capillary plexuses)VFS were higher in diabetic retinopathy compared with normal eyes. Elevated deep capillary plexusVFS correlated with decreased deep capillary plexus vessel density in nonproliferative diabetic retinopathy. Conclusion: Variable interscan time a