A suppression thermo-interlaced PCR method for the amplification of long DNA sequences was developed in this study using PCR suppression to repress non-specific products and nested thermo-interlaced cycling to optimize strand extension of target sequences. This method enables efficient and specific amplification of very long DNA sequences from complex genomes and synthetic DNA pools and has many…

The role of horizontal gene transfer in the evolution of multicellular eukaryotes such as plants and animals has long been elusive. This study identifies two major episodes of horizontal gene transfer in the evolution of land plants. Genes acquired from environmental organisms have fundamentally shaped the structure, physiology, and development of land plants.

Salicylic acid (SA) plays a pivotal role in plant response to biotic and abiotic stress. This study traces the origin and evolutionary trajectory of core SA signaling regulators and key proteins in SA biosynthesis. The study also reveals that the extensive presence of SA in green plant lineages is crucial for plant adaptation to high-irradiance environments.

In this study, the authors find that submerging intact seedlings promotes auxin-induced callus formation in shoot explants. Short-term submergence activates EIN2-dependent ethylene signaling, which promotes miR393 degradation. This process induces accumulation of the transcripts encoding TIR1/AFB2 auxin receptors, resulting in enhanced auxin responses. These findings provide a potential method…

Plants usually keep resistance (R) proteins in a static state under normal conditions to avoid autoimmunity and save energy for growth, but R proteins can be rapidly activated upon perceiving pathogen invasion. In this study, the authors find that SH3P2, an SH3 domain-containing protein that interacts with both Pib and AvrPib, suppresses effector-triggered, Pib-mediated immunity in rice,…

This study provides a modified protocol for root nodule protoplast preparation and single-cell RNA sequencing profiling of the indeterminate Medicago truncatula nodule. Based on the scRNA-seq data, the nodule cells can be classified into 13 clusters, and further detailed analyses of genes expressed in the symbiotic and un-symbiotic fate cells reveals that they have their distinct developmental…

This work reports an integrated and user-friendly toolkit, WGDI,for polyploid inference, hierarchical inference of genomic homology, and ancestral chromosome karyotyping. WGDI can improve the detection of whole-genome duplication and facilitates dynamic illumination of karyotype evolution.