Peptide-scFv antigen recognition domains effectively confer CAR T cell multiantigen specificity
Zoine et al. engineer a peptide-scFv antigen recognition domain as part of a bispecific chimeric antigen receptor (CAR). They utilize a systematic approach to design and test combinations by performing structural and functional evaluation. Their bispecific CAR demonstrates antitumor activity in vitro and in vivo while maintaining dual-antigen specificity.