Novel plant-derived bioactive compounds shape the human gut microbiome in vitro
Mounting evidence supports the potential of dietary bioactive compounds to reduce chronic disease risk. Recently, the biological activity of N-trans-caffeoyltyramine (NCT) and N-trans-feruloyltyramine (NFT) has been hypothesized to drive regulation of gut permeability,but the impact of these components on the human gut microbiome composition has not been studied. The aim of this work is to determine whether purified NCT and NFT, or a hemp hull product containing NCT and NFT (Brightseed(R) Bio Gut Fiber), can impact the gut microbiome using an in vitro fermentation assay. To address this question, we treated three fecal inocula, representative of the human gut microbiome, with Bio Gut Fiber(TM) and NCT/NFT and evaluated their respective impact against starch and methylcellulose as controls. We found strong changes exerted by Bio Gut Fiber(TM) and NCT/NFT on the gut microbiome relative to starch and methylcellulose, with distinct responses across all microbial communities. Among communit