In-silico study of fatty acid biosynthesis pathway enzymes in microalga Scenedesmus
Scenedesmus quadricauda is an important, rapidly growing, freshwater microalga explored as a source of alternative fuel because of its significantly high lipid content. However, the molecular basis of fatty acid biosynthesis is scarcely elucidated not only in S.quadricauda but also in Scenedesmus as a whole. This study aims to understand the 3D model structure of enzymes involved in fatty acid synthesis, and their catalytic sites compared to other Scenedesmus species. The first genome sequence S. quadricauda was carried initially in our isolate LWG002611 (GenBank ID NNCB00000000). However, till date, no study has been carried out on the 3D modelling and identifying the catalytic sites of fatty acid biosynthesis pathway enzymes. Mining the genome sequence of our isolated S. quadricauda LWG002611 as well as other Scenedesmus sequences taken from PhycoCosm and NCBI, we identified sequences of the crucial enzymes involved in fatty acid biosynthesis pathways such as acetyl-CoA carboxylase (