A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

A ratiometric genetically encoded voltage indicator (GEVI) would be desirable for tracking transmembrane voltage changes in cells that are undergoing motion. To create a high-performance ratiometric GEVI, we explored the possibility of adding a voltage-independent red fluorophore to ASAP3, a high-gain green fluorescent GEVI. We performed combinatorial multi-site mutagenesis on the cyan-excitable…

Read the full article here

Source: bioRxiv
Categories: General Medicine Journals and Societies, Latest Headlines
Tags:
Tweets with this article
bioRxiv
A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3 https://t.co/OZNHfwvJYS #bioRxiv

Related Articles