The transformation of fibroblasts into epithelial cells is critical for iPSC reprogramming. In this report, we describe studies with PFI-3, a small molecule inhibitor that specifically targets the bromodomains of SMARCA2/4 and PBRM1 subunit of SWI/SNF complex, as an enhancer of iPSC reprogramming efficiency. Our findings revealed that PFI-3 induces cellular plasticity in multiple human dermal…

Maize is currently the most productive cereal crop in the world (www.faostat.org). It can associate with the Arbuscular Mycorrhizal Fungus (AMF) Rhizophagus irregularis which can provide additional water and mineral nutrients to the plant in return for C delivered by the host plant. Two maize lines were characterized at the physiological and molecular levels as they displayed contrasting…

Alz-5 acts as a bifunctional chelator that can interact with various Ab aggregates and reduce their neurotoxicity. Single-cell ROS measurements provided by Pt-nanoelectrode technique revealed a significant antioxidant properties of Alz-5 in Ab42 – affected SH-SY5Y cell. AFM data obtained on Ab42 fibrils clearly indicate an anti-aggregating property of Alz-5. Young modulus mapping on living…

To verify the parasitic lifestyle of Candidatus Patescibacteria in the enrichment cultures derived from a methanogenic bioreactor, we applied multifaceted approaches combining cultivation, microscopy, metatranscriptomic, and protein structure prediction analyses. Cultivation experiments with the addition of exogenous methanogenic archaea with acetate, amino acids, and nucleoside monophosphates…

Focal adhesions (FAs) form the junction between extracellular matrix (ECM)-bound integrins and the actin cytoskeleton and also transmit signals that regulate cell adhesion, cytoskeletal dynamics, and cell migration. While many of these signals are rooted in reversible tyrosine phosphorylation, phosphorylation of FA proteins on Ser/Thr residues is far more abundant yet its mechanisms and…

Rare cell populations are key in neoplastic progression and therapeutic response, offering potential intervention targets.However, their computational identification and analysis often lag behind major cell types. To fill this gap, we introduced MarsGT: Multi-omics Analysis for Rare population inference using Single-cell Graph Transformer.It identifies rare cell populations using a…

This research focuses on the investigation of the propagation of frequencies between 0.1 and 2.5 THz through a phantom ear model using terahertz (THz) time-domain spectroscopy (TDS). While the use of THz frequencies between 0.1 to 0.3 THz in fifth and sixth generation cellular networks has gained significant attention, there is also a growing interest in utilising higher frequencies, such as 1…

In the following research, a novel antifungal culture method was discussed with the aim of developing bacterial culture media in which fungal growth is inhibited and bacterial growth is stimulated. This technique is based on the use of lactic acid as an antifungal agent, due to its capacity to neutralise the electrochemical gradient of fungal cell membranes, thus inhibiting their development….

When a population is isolated and composed of few individuals, genetic drift is the paramount evolutionary force. Inbreeding might also occur, resulting in genomic regions that are identical by descent, manifesting as runs of homozygosity (ROHs) and the expression of recessive traits; accordingly, ROH can be used to map phenotypic traits. Likewise, the genes underlying traits of interest can be…