3D-cultured blastoids model human embryogenesis from pre-implantation to early gastrulation stages
Theunissen et al. describe an efficient protocol for generating blastoids from naive human pluripotent stem cells and allowing their extended culture on 3D matrices for up to 21 days. This system offers a continuous and integrated model of human embryonic and extraembryonic development from pre-implantation to early gastrulation stages.