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Mashup Score: 4Evaluating metagenomics and targeted approaches for diagnosis and surveillance of viruses - Genome Medicine - 4 hour(s) ago
Background Metagenomics is a powerful approach for the detection of unknown and novel pathogens. Workflows based on Illumina short-read sequencing are becoming established in diagnostic laboratories. However, high sequencing depth requirements, long turnaround times, and limited sensitivity hinder broader adoption. We investigated whether we could overcome these limitations using protocols based on untargeted sequencing with Oxford Nanopore Technologies (ONT), which offers real-time data acquisition and analysis, or a targeted panel approach, which allows the selective sequencing of known pathogens and could improve sensitivity. Methods We evaluated detection of viruses with readily available untargeted metagenomic workflows using Illumina and ONT, and an Illumina-based enrichment approach using the Twist Bioscience Comprehensive Viral Research Panel (CVRP), which targets 3153 viruses. We tested samples consisting of a dilution series of a six-virus mock community in a human DNA/RNA ba
Source: genomemedicine.biomedcentral.comCategories: General Medicine News, Infectious DiseaseTweet
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Mashup Score: 37
We may have an answer to the “mystery illness” in the Democratic Republic of Congo. Here’s what the story reveals about America’s global health commitments.
Source: www.statnews.comCategories: General Medicine News, Infectious DiseaseTweet-
@statnews “In a world where pathogens are constantly emerging and evolving, we must have systems in place to rapidly detect, investigate, and respond — especially when it’s not a familiar foe.” This is what biocontainment labs do. And why they’re critical. https://t.co/Z0gFVQMl2x
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Mashup Score: 5How rich musicians billed American taxpayers for luxury hotels, shopping sprees, and million-dollar bonuses - 8 hour(s) ago
Pop stars took advantage of the SVOG grant, using COVID relief funds for luxury spending. Lil Wayne bought Balenciaga and flew private.
Source: www.businessinsider.comCategories: General Medicine News, Infectious DiseaseTweet-
How rich musicians billed American taxpayers for luxury hotels, shopping sprees, and million-dollar bonuses https://t.co/rL4mwDxjzK
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Mashup Score: 3Chris Brown, Lil Wayne Spent Covid Relief Aid on Parties, Private Jets, and More: Report - 8 hour(s) ago
A new report from ‘Insider’ reveals how major artists like Lil Wayne, Chris Brown, and Alice in Chains spent the millions they received in Covid aid.
Source: www.rollingstone.comCategories: General Medicine News, Infectious DiseaseTweet-
Lil Wayne, Chris Brown Spent Covid Aid on Parties, Jets: Report https://t.co/GfBhAMBnvC
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Mashup Score: 21Laboratory Biosafety Guidelines for working with SARS-CoV-2 - 9 hour(s) ago
Provides general and specific biosafety laboratory guidelines for handling SARS-CoV-2 specimens
Source: www.cdc.govCategories: General Medicine News, Infectious DiseaseTweet-
Updated - Lab Biosafety Guidelines for working with SARS-CoV-2 | “At a minimum, BSL-2 facilities, practices, and procedures are recommended for diagnostic research, anatomic pathology, environmental testing, and virus propagation utilizing SARS-CoV-2.“ https://t.co/0NVS2DS52T
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Mashup Score: 4Linkage-based ortholog refinement in bacterial pangenomes with CLARC - 13 hour(s) ago
Bacterial genomes exhibit significant variation in gene content and sequence identity. Pangenome analyses explore this diversity by classifying genes into core and accessory clusters of orthologous groups (COGs). However, strict sequence identity cutoffs can misclassify divergent alleles as different genes, inflating accessory gene counts. CLARC (Connected Linkage and Alignment Redefinition of COGs) (https://github.com/IndraGonz/CLARC) improves pangenome analyses by condensing accessory COGs using functional annotation and linkage information. Through this approach, orthologous groups are consolidated into more practical units of selection. Analyzing 8,000+ Streptococcus pneumoniae genomes, CLARC reduced accessory gene estimates by more than 30% and improved evolutionary predictions based on accessory gene frequencies. By refining COG definitions, CLARC offers critical insights into bacterial evolution, aiding genetic studies across diverse populations. ### Competing Interest Statement
Source: www.biorxiv.orgCategories: General Medicine News, Infectious DiseaseTweet-
Excited to share a new preprint led by Indra Gonzalez Ojeda on a new tool to improve pangenome calling in bacteria, CLARC. https://t.co/BAnSAaFf26 . Passing output of Roary for S. pneumoniae populations through this tool reduces accessory genome > 30%, resolves other puzzles
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Mashup Score: 7Elon Musk and SpaceX Face Federal Reviews After Violations of Security Reporting Rules - 13 hour(s) ago
Federal agencies have opened at least three reviews into whether the company and its leader complied with disclosure protocols intended to protect state secrets, people with knowledge of the matter said.
Source: www.nytimes.comCategories: General Medicine News, Infectious DiseaseTweet-
Elon Musk and SpaceX Face Federal Reviews After Violations of Security Reporting Rules - The New York Times https://t.co/mOcF6upc7p
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Mashup Score: 5Minocycline-Induced Cutaneous Hyperpigmentation in an Orthopedic Patient Population - 13 hour(s) ago
Abstract. Background. The objectives of this study were to estimate the incidence and evaluate risk factors for development of minocycline-induced cutaneou
Source: academic.oup.comCategories: General Medicine News, Infectious DiseaseTweet-
The correct answer is minocycline. Study of 291 patients: 54% developed hyperpigmentation, most commonly blue-gray discoloration in lower extremities, at mean duration 1.5 years. Risk factors reported in this study: https://t.co/MHr8AswnOA https://t.co/IVpSwE8hOC
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Mashup Score: 1
The data shows that RNA interacts with a mitochondrial protein complex to trigger immune response pathways when viral RNA is present.
Source: www.genengnews.comCategories: General Medicine News, Infectious DiseaseTweet-
“While protein-protein interactions and protein modifications are critical for antiviral signaling through the MAVS signalosome, our research highlights the significant role of cellular RNA molecules in this process” https://t.co/7uf2iDVOxV
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Mashup Score: 12Rwanda's Marburg outbreak declared over - 14 hour(s) ago
The case-fatality rate was about 23%, which is at the low end of the 24% to 88% range.
Source: www.cidrap.umn.eduCategories: General Medicine News, Infectious DiseaseTweet-
Rwanda's Marburg outbreak declared over The case-fatality rate was about 23%, which is at the low end of the 24% to 88% range. https://t.co/Z9RgkxiKYQ Photo: NIAID/Flickr cc https://t.co/5wAzhlHUn8
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Very nice work: metagenomic sequencing is a catch all technique for pathogen detection. This study does extensive testing of a nanopore based protocol https://t.co/mFQeGBWRbm