A Functional sgRNA-CRISPR screening method for generating murine RET and NTRK1 rearranged oncogenes – PubMed

CRISPR/Cas9 gene editing represents a powerful tool for investigating fusion oncogenes in cancer biology. Successful experiments require that sgRNAs correctly associate with their target sequence and initiate double stranded breaks which are subsequently repaired by endogenous DNA repair systems yie …

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