• Mashup Score: 0
    ISHLT: The International Society for Heart & Lung Transplantation - Journal Watch - 6 month(s) ago

    Accept Cookies & Privacy Policy? This website uses cookies to ensure the best possible web experience. By continuing and using the site, you consent to the use of cookies. If you wish to disable them or to learn more about how we use cookies, please view our Cookies Policy. …

    Source: www.ishlt.org
    Categories: Cardiology News and Journals, Latest Headlines
    Tweet Tweets with this article
    • Profile photo of ISHLT
      ISHLT

      🫀🦠🫁 October #ISHLTJournalWatch is out w/#Pathology #Transplantation topics! Issue edited by Federica Pezzuto, & feat. contributors @NCortesMD, Alessia Giarraputo, Maja Nackenhorst, and Alexander Wein. Thanks to senior editor @avfDO. 🔖 Read now: https://t.co/hXAiaUj3II #ISHLT https://t.co/2nuorZypQO

  • Mashup Score: 2
    Droplet digital PCR (ddPCR) using FFPE DNA to assess methylation status of MGMT gene among patients with IDH mutant astrocytoma and IDH wild-type glioblastoma - 6 month(s) ago

    MGMT promoter methylation analysis in formalin-fixed paraffin-embedded (FFPE) tissues can be challenging since the DNA obtained is often fragmented. Bisulfite conversion, which is essential to determine methylation status, further degrades DNA. While conventional methylation-specific PCR (MSP) and pyrosequencing assays have long been used to determine the methylation status of MGMT , this study was designed to determine the utility of one-tube DNA extraction method coupled with a droplet digital PCR (ddPCR) assay, to study the epigenetic changes in the promoter region of the MGMT gene using DNA obtained from FFPE. The FFPE blocks of 30 (n=30) patients with Central Nervous System (CNS) WHO grade 4 tumours, previously tested by MSP (2011–2021) were retrieved; DNA was extracted using one-tube extraction method and bisulfite converted. All converted samples were analyzed for methylation status of the MGMT promoter region with a laboratory designed Methylation-Specific ddPCR (MS ddPCR) usin

    Source: jcp.bmj.com
    Categories: General Medicine Journals and Societies, Latest Headlines
    Tweet Tweets with this article
    • Profile photo of JClinPath_BMJ
      JClinPath_BMJ

      🎯🧬 MS ddPCR: High-sensitivity MGMT methylation in FFPE. 💲🔬 Budget-friendly vs. MSP. 🌟🎯 Boosts glioblastoma treatment accuracy. ✅👩‍🔬 Easy & routine-friendly. 🌈 #Oncology #Pathology #MGMT #FFPE #ddPCR #CostEffective 🌈 https://t.co/tBaH5DoVxJ

  • Mashup Score: 0
    Preference of grade and lymphovascular invasion over invasive size measurement in stage I lung adenocarcinoma - 6 month(s) ago

    Aims Although it is necessary to measure the invasive size of lung adenocarcinoma with a lepidic component, it is not uncommon to have trouble in measuring the invasive size of lung adenocarcinoma. This study examined whether there were other stronger prognostic factors than invasive size. Methods We characterised the clinicopathological features associated with recurrence-free survival (RFS) of 686 patients with the pathological stage (p-Stage) I lung adenocarcinoma. Moreover, we compared the area under the curve (AUC) values for recurrence between various combinations of pathological-baseline (age & sex & p-Stage based on invasive size) (B(i)) and several prognostic factors, and various combinations of p-baseline based on total tumour size (B(t)) and several prognostic factors. Results AUC showed no significant differences between B(i) & new International Association for the Study of Lung Cancer grade (G) or vascular invasion (V), and B(t) & G or V. AUC was the highest in B & G & lym

    Source: jcp.bmj.com
    Categories: General Medicine Journals and Societies, Latest Headlines
    Tweet Tweets with this article
    • Profile photo of JClinPath_BMJ
      JClinPath_BMJ

      🧐 Stage 1 lung cancer: grade, lymphovascular invasion more powerful prognostic factors than size 🙀 Recurrence-free survival worse with: ❌G3 ❌Lymphovascular invasion For stage I, focus on grade & invasion, not just size 👀 #pathology https://t.co/8TNQmDQz8H https://t.co/AFrV3ykLdu

  • Mashup Score: 4
    Droplet digital PCR (ddPCR) using FFPE DNA to assess methylation status of MGMT gene among patients with IDH mutant astrocytoma and IDH wild-type glioblastoma - 6 month(s) ago

    MGMT promoter methylation analysis in formalin-fixed paraffin-embedded (FFPE) tissues can be challenging since the DNA obtained is often fragmented. Bisulfite conversion, which is essential to determine methylation status, further degrades DNA. While conventional methylation-specific PCR (MSP) and pyrosequencing assays have long been used to determine the methylation status of MGMT , this study was designed to determine the utility of one-tube DNA extraction method coupled with a droplet digital PCR (ddPCR) assay, to study the epigenetic changes in the promoter region of the MGMT gene using DNA obtained from FFPE. The FFPE blocks of 30 (n=30) patients with Central Nervous System (CNS) WHO grade 4 tumours, previously tested by MSP (2011–2021) were retrieved; DNA was extracted using one-tube extraction method and bisulfite converted. All converted samples were analyzed for methylation status of the MGMT promoter region with a laboratory designed Methylation-Specific ddPCR (MS ddPCR) usin

    Source: jcp.bmj.com
    Categories: General Medicine Journals and Societies, Latest Headlines
    Tweet Tweets with this article
    • Profile photo of JClinPath_BMJ
      JClinPath_BMJ

      🎯🧬 MS ddPCR: High-sensitivity MGMT methylation in FFPE. 💲🔬 Budget-friendly vs. MSP. 🌟🎯 Boosts glioblastoma treatment accuracy. ✅👩‍🔬 Easy & routine-friendly. 🌈 #Oncology #Pathology #MGMT #FFPE #ddPCR #CostEffective 🌈 https://t.co/tBaH5DoVxJ

Load More