Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems

Yang et al. show that the dCas13 system is capable of labeling RNAs. Applying orthogonal dCas13s or combining with dCas9 allows simultaneous visualization of RNA-RNA and DNA-RNA in living cells. The dCas13 system is user friendly in real-time RNA imaging without requiring genetic manipulation.

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